Intermediate

Part:BBa_K1904001

Designed by: Roger Milton Rubio S�nchez, Enrique Chimal, Enrique Palomino, Brian Morteo   Group: iGEM16_TecCEM   (2016-06-06)

Endolysin with RBS and terminator. N-terminal HisTag

This biobrick is comprised by the mutated endolysin (BBa_K1904000) assembled with B0034 (RBS) and B0015 (double terminator), all in RFC10 standard. This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. This part was complemented with a N-terminal HisTag in order to allow its purification via a Nickel-affinity chromatography.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None